Expanded programme on immunization

2nd Polio Laboratory Network Training Workshop to Implement the Real-time Polymerase Chain Reaction Techniques for Rapid Detection and Characterization of Polioviruses

13-17 January 2014 - Beijing, China

WHO/Y. Jee


A five-day hands-on training workshop to implement real-time PCR for polio ITD and VDPV screening was organized in Beijing China on 13-17 January 2014 for the remaining China provincial laboratories and the national polio laboratories in Mongolia and Malaysia. Ten participants representing eight China provincial laboratories (Chongqing, Guizhou, Hainan, Hubei, Inner Mongolia, Liaoning, Ningxia and Qinghai) and two national polio laboratories (Mongolia and Malaysia) were trained. There were also 20 observers from 16 provincial laboratories and four from China CDC who attended the training workshop. Laboratory experts from Victorian Infectious Diseases Reference Laboratory, Melbourne (WHO regional reference laboratory for poliomyelieis) and WHO Regional Office for the Western Pacific participated in this training as facilitators.


The objectives of the workshop is to improve the poliovirus real-time ITD technique of laboratory staff in China, Malaysia and Mongolia and to introduce the standard operating procedure of new algorithm for poliovirus detection in polio laboratory network in the Western Pacific Region.


The Western Pacific Region is making efforts to reduce the time to identify polioviruses by introducing real-time polymerase chain reaction (PCR) for polio intratypic differentiation (ITD) and vaccine-derived poliovirus (VDPV) screening among polio network laboratories including provincial laboratories in China. In March 2012, 23 China provincial laboratories were trained on real-time PCR for polio ITD and VDPV screening and 22 provincial laboratories successfully implemented the real time PCR techniques by January 2013.

The objectives of the workshop were achieved through intensive hands-on practical sessions and lectures. During the workshop, implementation steps were discussed and it is expected that these laboratories will conduct real-time PCR for polio ITD and VDPV screening and after successful completion of implementation steps including proficiency test. After the successful implementation of ITD, those laboratories will be upgraded to polio ITD laboratories and will be assessed for ITD function during the WHO annual accreditation visits.


This article also appears on the Global Immunization Newsletter (GIN), accessible from the link below.